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KMID : 0359320080480040393
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Korean Journal of Veterinary Research
2008 Volume.48 No. 4 p.393 ~ p.399
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Radioprotective effect of fucoidan against hematopoietic and small intestinal stem cells of γ-ray irradiated mice
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Park Eun-Jin
Jeon Seong-Mo
Joo Hong Gu
Hwang Kyu-Kye
Jee Young-Heun
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Abstract
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We investigated the potential of fucoidan for its ability to provide protection from gamma ray-induced damage. In our results, the fucoidan significantly improved the counts of endogenous colony forming unit to 9.5±1.5, from 5.5±2.5 compared with un-treated irradiated control group at 10 day after 7 Gy whole body irradiation. After 2 Gy irradiation, fucoidan treatment attenuated the percent of tail DNA of splenocytes, parameters of DNA damage, from 30.17±1.7% to 13.67±2.81% by comet assay and also accelerated the proliferation of splenocytes, compared with un-treated irradiated control group by 3H-thymidine incorporation assay. Furthermore, fucoidan decreased the number of apoptotic fragments per intestinal crypt by 31.8% at 1 days after 2 Gy irradiation. These results indicated that the fucoidan significantly improved the hematopoietic recovery, prevented the DNA damage in immune cells and enhanced their proliferation, which had been suppressed by ionizing radiation. in addition, fucoidan rescued intestinal cells from radiation-induced apoptosis. Thus, this study raises the possibility of using fucoidan as adjuvant therapeutic agent after radiotherapy.
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KEYWORD
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apoptosis, fucoidan, gamma-ray, hematopoiesis, mice
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